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The British military’s United Kingdom Maritime Trade Operations center reported the attack, saying the ship’s crew was safe and no environmental damage was reported.
No one immediately claimed the attack. However, Iran attacked a ship Thursday off Oman trying to get out of the Arabian Gulf.
The strikes show the danger of the Iran war again spinning out of control, even after Iran and the US reached an interim deal to try and reach a final accord to end the conflict.
The US military has conducted strikes on Iranian targets after President Donald Trump accused Iran of a "foolish violation" of its truce following an attack on a cargo ship in the Strait of Hormuz.
US Central Command said it had struck missile and drone storage facilities and coastal radar positions on Friday, in response to a drone attack on a cargo ship on Thursday which halted a planned evacuation of sailors stuck in the region.
Tehran said the cargo ship was attacked because it was using an unauthorised route to transit through the Gulf waterway.
After the US strikes, Iran in turn accused the US of violating their interim deal and said it had struck targets linked to American forces.
US Central Command - or Centcom - described the American strikes as "a powerful response" to the drone attack a day earlier.
"The unwarranted aggression against commercial shipping by Iranian forces clearly violated the ceasefire," it said in a statement.
"Furthermore, Iran's dangerous behaviour undermined freedom of navigation as commerce increasingly flows through the vital international trade corridor."
Centcom said the US military would "continue to provide safe passage coordination and support to commercial vessels transiting the strait".
Iran's foreign ministry released a statement on Saturday morning, saying the country had carried out strikes against targets linked to American forces in response, and blamed the "treaty-breaking US regime" for the situation.
Iran's Islamic Revolutionary Guard Corps (IRGC) said its navy had struck US military positions in the region, without providing further details. The BBC contacted the Pentagon for comment.
Bahrain's foreign ministry said the country had come under attack from "several Iranian drones" early on Saturday, condemning the action as a "flagrant violation" of its sovereignty and accusing Tehran of undermining peace efforts.
Also on Saturday, the United Kingdom Maritime Trade Operations (UKMTO) said a tanker was struck by an unidentified projectile in the Strait of Hormuz.
The vessel sustained damage to its bridge, but all crew were safe and no environmental damage had been reported, UKMTO added.UN nuclear chief says inspectors will visit Iran sites as part of war deal
Tehran effectively closed the strait after US and Israeli attacks against Iran began at the end of February.
The shutdown of the critical waterway for oil and gas shipments caused a spike in global oil prices and choked off shipments of other crucial commodities such as fertiliser.
The US and Iran agreed on 17 June to end hostilities under a 14-point memorandum of understanding, which had also called for Iran to use its "best efforts for the safe passage of commercial vessels with no charge for 60 days".
In a post on X following the US retaliatory strikes, Vice-President JD Vance said that if Iran "has disagreements about how the MOU is being applied, they can pick up the phone".
"But violence will be met with violence," he added.Ebrahim Azizi, head of the Iranian parliament's national security commission, though, said on social media that the US had "attacked Iran in the middle of negotiations once again".
He continued in his social media post: "This reckless violation of the ceasefire will, as always, lead to retreat and regret on their part. The blame game does not work anymore."
Speaking to reporters at the White House on Friday afternoon, Trump refused to be drawn into questions on how the US might respond to the drone attack, or whether he viewed the ceasefire as still intact.
"You'll find out," he said. "I don't like the fact that they took a shot yesterday. They shouldn't be doing that."
Asked why he believed Iran would conduct such an operation, Trump said only that "they're a little bit different".
Bahrain said Saturday that Iran launched a drone attack on the island kingdom just after Tehran said it targeted American military installations to retaliate for overnight airstrikes.
The strikes on Bahrain show the danger of the Iran war again spinning out of control, even after Iran and the U.S. reached an interim deal to try and reach a final accord to end the conflict.
The US had launched its airstrikes in response to an Iranian drone attack on a ship trying to get out of the Strait of Hormuz on Thursday, continuing a string of attacks that have shaken the uneasy ceasefire in the war.
A maritime body overseen by the US Navy said Saturday that a route through the Strait of Hormuz near Oman’s shores is expanding to allow for both inbound and outbound traffic.
The announcement by the Joint Maritime Information Center serves as another warning to Iran that the US is pushing to reopen the strait.
Iran has insisted ships must obey its orders and is warning it will start charging fees for transit through the strait, through which a fifth of all oil and natural gas once passed.
The US and Gulf Arab states have rejected Iran’s demands. The strait is considered around the world as an international waterway, despite being the territorial waters of Iran and Oman.
In recent days, Trump and other US officials insisted negotiations with Iran were progressing well, saying Iran had given up any suggestion of tolling vessels transiting through the Strait of Hormuz.
In a Truth Social post on Wednesday, Trump said Iran had informed the US that there would be "no tolls, no insurance costs and no other charges of any kind being sought or received".
"If this is false information, negotiations would end, immediately," he added.
The US has condemned reports that Iran is charging fees to tankers going through the strait, and many see any tolling system as breaking with international maritime law.
On Tuesday, Iranian and Omani officials held talks in Oman's capital of Muscat to discuss "the future management of navigation", although Omani Foreign Minister Badr Al-Busaidi said both countries were committed to "toll-free safe passage".
However, Iran's chief negotiator, Mohammed Bagher Ghalibaf, told state-affiliated news outlets that "everyone should know that the administration of the Strait of Hormuz will never go back to the way it was before the war."
The cargo ship hit by a projectile on Thursday was the Ever Lovely, a Singapore-flagged vessel.
According to British maritime security agency UKMTO, the ship was struck 7.5 nautical miles south-east of Oman's port of Dahit.
The Ever Lovely had been following the UKMTO's recommended route through the strait when it was struck, the ship's owner, Evergreen, said.
"All crew members remain safe as does the vessel itself and all cargo," it added.
In response, the UN's International Maritime Organization (IMO) paused its planned evacuation of more than 11,000 sailors who have been stranded in the key shipping lane since the war erupted.